Light sheet microscopy illuminates only a thin plane of the sample at a time, dramatically reducing photodamage while enabling long-term time-lapse imaging in 3D. By separating illumination and detection paths, light sheet systems minimize excitation light exposure compared to confocal methods. Lattice light sheet microscopy takes this further with an ultra-thin sheet of light, allowing researchers to observe delicate live samples over extended periods with minimal bleaching or toxicity.
Our facility features the 3i Lattice Light Sheet Microscope for ultra-gentle, high-speed 4D imaging.
Benefits of light sheet approaches:
- Extremely low phototoxicity, ideal for live imaging
- Fast, volumetric data acquisition
- High spatial and temporal resolution
- Compatible with a wide range of biological samples
- 3i Lattice Light Sheet Microscope
- An extremely gentle microscope that generates an optical lattice to create an ultra-thin light sheet to image biological samples over long periods of time and with very fine resolution. This allows for 4D live cell imaging, where experiments limited to seconds or minutes on other imaging platforms can be extended to hours or even days.